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Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

Current location：HOME>PRODUCT>Microplate Reader>Multifunctional Microplate Reader

Multifunctional Microplate ReaderYP-96-T

【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of Multifunctional Microplate Reader, with custom services. Complete Multifunctional Microplate Reader models, high detection accuracy, trusted by customers.

+86 19963093538

Product Details

I. Instrument Introduction

The Youyunpu Series Multifunctional Microplate Analyzer is a high‑sensitivity, high‑dynamic‑range and high‑linearity microplate reader designed with innovative linear PMT detection technology and dual‑excitation light source compensation technology.

With different configurations, it supports three detection modes: Chemiluminescence (CLIA), Light‑Initiated Chemiluminescent Assay (LiCA) and Time‑Resolved Fluorescence (TRF).

Users can flexibly configure 1 to 3 modes, providing a highly adaptable solution for researchers.

The instrument is widely used in nucleic acid, immunoassay and cell analysis, including protein detection, enzyme kinetics, kinase assays, molecular interaction, agglutination, reporter gene assays, cell proliferation, apoptosis, cytotoxicity, bacterial adhesion, food toxin detection and hormone testing.

Chemiluminescence (CLIA)

Photons generated by chemiluminescent reactions are converted into electrical signals by a photomultiplier tube (PMT) for detection. The instrument collects signals across the full spectrum.

Chemiluminescence is highly valued for its high sensitivity and wide dynamic range. It is optically simpler than fluorescence, requiring no excitation source or dedicated optics.

The system supports glow‑type chemiluminescence, cytotoxicity and proliferation assays.

Light‑Initiated Chemiluminescent Assay (LiCA)

LiCA is a homogeneous affinity detection method based on bead pairs, suitable for almost all research applications.

This wash‑free assay enables simple, rapid and highly sensitive detection of biomolecules in cell lysates, supernatants, serum and other sample types, as well as binding assays with a wide affinity range.

LiCA completes 96‑well plate measurements in minutes with an extremely high signal‑to‑background ratio, enabling fast, high‑sensitivity detection in almost any laboratory.

Time‑Resolved Fluorescence (TRF)

TRF provides high signal‑to‑noise ratio, excellent sensitivity, wide dynamic range, outstanding stability and flexibility, even with limited or ultra‑low‑abundance samples.

Similar to conventional ELISA, TRF uses lanthanide chelate fluorescence delay to reduce background interference, expand dynamic range and deliver highly stable signals.

II. Instrument Features

Dual‑mode photon counting and correction technology
Patented linear fitting technology expands the dynamic range from 10⁵ to 10⁷ (100× extension), greatly improving linearity for wide concentration gradients.
Confocal optical system
Effectively suppresses ambient stray light and minimizes cross‑talk between adjacent wells caused by large concentration differences.
Optimized optics for each mode

In TRF mode: dual excitation paths + high‑dynamic‑range PMT detect weak signals without saturating strong signals, maximizing dynamic range and linearity.
In LiCA mode: monochromatic laser excitation minimizes non‑specific fluorescence from the microplate or reaction mixture.

High stability & temperature control
Long‑life monochromatic LEDs and laser diodes ensure reliable performance and repeatability.
Built‑in temperature control up to 45 °C with high uniformity ensures consistent reactions and well‑to‑well reproducibility.
User‑friendly software
Intuitive workflow interface with preloaded protocols and templates for routine applications.
Supports standard curve quantitation, endpoint, kinetic and slope measurement modes.
Provides multiple curve‑fitting models for quantification, calibration and data analysis.
Self‑diagnosis & auto‑calibration
Power‑on self‑test and automatic calibration; auto dynamic range adjustment simplifies parameter setup.
Supports 8×12 opaque microplates with automatic tray loader, enabling integration with liquid handling systems.
Compact, lightweight design saves laboratory space.

III. Specifications

Item	Specification
Model	YP‑96‑L, YP‑96‑T, YP‑96‑CLIA
Microplate	96‑well opaque microplate
Reading Mode	Top reading
Temperature Control	Ambient to 45 ℃
Supported Assays	LiCA, TRF, Glow‑type CLIA
Excitation Wavelength	685±5 nm (LiCA); 365±10 nm (TRF); N/A (CLIA)
Emission Wavelength	600–620 nm (LiCA); 610±10 nm (TRF); 380–700 nm (CLIA)
Dark Background	< 200 CPS
Sensitivity	100 pg/mL
Repeatability	< 3%
Dynamic Range	7 orders of magnitude
Measurement Modes	Endpoint, Slope, Kinetic
Curve Fitting	Linear, Logarithmic, Exponential, Polynomial, 4‑Parameter Logistic (4PL)
Other Functions	Programmable modes, automatic tray loader
Weight	9 kg
Dimensions	345 mm × 248 mm × 210 mm
Power	AC 220 V, 50/60 Hz