I. Product Overview

The YP-P08 Gene Amplification Instrument is mainly used to perform real-time fluorescent quantitative PCR experiments. Through fluorescence excitation and collection, it monitors the experimental process in real time, plots the fluorescence curve from the process data, displays it on the instrument interface in real time, fits and analyzes the experimental data, and finally generates an experimental report in PDF format.

It performs rapid and accurate qualitative and quantitative analysis of target nucleic acids in biological samples taken from other animals (such as blood, body fluids, etc.), and can also analyze special samples for standard curves, melting curves, genotyping, etc.

II. Basic Principle

Principle of the YP-P08 Gene Amplification Instrument: Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro. A temperature cycle consists of several steps: high-temperature denaturation, low-temperature annealing, and appropriate-temperature extension, enabling rapid amplification of the target gene. It features strong specificity, high sensitivity, simple operation and time saving, representing a major innovation in gene amplification technology. PCR technology can specifically amplify trace amounts of target DNA by millions of times, greatly improving the ability to analyze and detect DNA molecules.

Real-time fluorescent quantitative PCR technology adds corresponding fluorescent dyes or fluorescent-labeled probes on the basis of conventional PCR. During the PCR reaction, the entire PCR process is detected in real time through changes in fluorescent signals. It is a method of monitoring the total product after each PCR cycle with fluorescent chemicals, and quantitatively analyzing specific DNA sequences in the test sample by internal or external reference methods.

III. Basic Instrument Structure

The Gene Amplification Instrument is a real-time reaction detection instrument, mainly composed of a gene amplification thermal cycling system, a real-time fluorescence detection system, a microcircuit control system, a computer and supporting application software. It has two core functional modules: the thermal cycling system and the real-time fluorescence detection system. The working principle of the gene amplification thermal cycling system is basically the same as that of traditional gene amplifiers, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly comprises fluorescence excitation components, optical signal transmission components, fluorescence detection components and a control system.

IV. Instrument Features

1. Small size, light weight and easy to carry, easily meeting the needs of field experiments.

2. Built-in 7-inch high-definition capacitive touch screen PDA, with touch operation that is simple and fast.

3. Marlow high-quality Peltier cooling chip, combined with high-end German PT1000 temperature sensor and electrical resistance heating compensation for edge temperature control mode, with a maximum heating rate of 7 °C/s and maximum cooling rate of 5 °C/s, greatly shortening the experimental time.

4. Whole-plate 3s rapid light collection mode, ensuring well position consistency of experimental results.

5. Simple and intuitive software guidance for easy start of detection experiments.

V. Application Fields

• Basic scientific research

• Pathogen detection

• Animal epidemic detection

• Meat product adulteration

• GMO detection

• Food hygiene and quarantine

• Drug development and rational drug use

• Gene expression

• Water monitoring

VI. Technical Parameters

• Sample Capacity: 8 × 0.2 mL, supports 8-strip tubes

• Compatible Consumables: Common transparent PCR consumables, 8 × 0.2 mL strip tubes, 0.2 mL single tubes

• Reaction System: 5–120 μL reaction volume

• Heating/Cooling Module: Imported semiconductor thermoelectric module

• Temperature Control Range: 4 °C–99 °C

• Average Heating/Cooling Rate: ≥ 2 °C/s

• Temperature Control Accuracy: ≤ ±0.1 °C

• Temperature Uniformity: ≤ ±0.2 °C

• Number of Temperature Control Zones: Multi-point (2 points)

• Number of Gradients: 0

• Gradient Temperature Range: None

• Number of Gradient Wells: None

• Excitation Light Source: Maintenance-free LED

• Excitation Wavelength Range: 400–700 nm

• Detection Component: Imported photodetector

• Number of Detection Channels: Standard 1 channel (FAM); High-end (optional): FAM, VIC

• Compatible Dyes and Probes: FAM/SYBR Green I, VIC/HEX/Cy3 (optional), ROX/Texas Red (optional), Cy5, TAMARA (optional)

• Software Functions: Real-time fluorescent quantitative PCR system software; real-time amplification reaction curve function; real-time reaction curve display for specific samples; data analysis function; automatic positive/negative result judgment function; graphical display function

• Noise: < 45 dB

• Screen Size: 7 inches (HD)

• Touch Screen: Capacitive

• External USB: Supports data import and export

• Hot Lid: Automatic pressure adjustment

• Dimensions (W×L×H): 290 × 308 × 130 mm

• Case Dimensions (L×W×H): 75 × 38 × 19 cm

• Net Weight: Approx. 3 kg