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Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

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PCR Thermal CyclerYP-P48

【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of PCR Thermal Cycler, with custom services. Complete PCR Thermal Cycler models, high detection accuracy, trusted by customers.

+86 19963093538

Product Details

I. Product Overview

The YP-P48 PCR Thermal Cycler is mainly used to perform real-time fluorescent quantitative PCR experiments. Through fluorescence excitation and collection, it monitors the experimental process in real time, plots the fluorescence curve from the process data, displays it on the instrument interface in real time, fits and analyzes the experimental data, and finally generates an experimental report in PDF format.

It performs rapid and accurate qualitative and quantitative analysis of target nucleic acids in biological samples taken from other animals (such as blood, body fluids, etc.), and can also analyze special samples for standard curves, melting curves, genotyping, etc.

II. Basic Principle

Principle of the YP-P48 PCR Thermal Cycler: Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro. A temperature cycle consists of several steps: high-temperature denaturation, low-temperature annealing, and appropriate-temperature extension, enabling rapid amplification of the target gene. It features strong specificity, high sensitivity, simple operation and time saving, representing a major innovation in gene amplification technology. PCR technology can specifically amplify trace amounts of target DNA by millions of times, greatly improving the ability to analyze and detect DNA molecules.

Real-time fluorescent quantitative PCR technology adds corresponding fluorescent dyes or fluorescent-labeled probes on the basis of conventional PCR. During the PCR reaction, the entire PCR process is detected in real time through changes in fluorescent signals. It is a method of monitoring the total product after each PCR cycle with fluorescent chemicals, and quantitatively analyzing specific DNA sequences in the test sample by internal or external reference methods.

III. Basic Instrument Structure

The PCR Thermal Cycler is a real-time reaction detection instrument, mainly composed of a gene amplification thermal cycling system, a real-time fluorescence detection system, a microcircuit control system, a computer and supporting application software. It has two core functional modules: the thermal cycling system and the real-time fluorescence detection system. The working principle of the gene amplification thermal cycling system is basically the same as that of traditional gene amplifiers, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly comprises fluorescence excitation components, optical signal transmission components, fluorescence detection components and a control system.

IV. Equipment Parameters

Item	Specification
Product Name	YP-P16 / YP-P32 / YP-P48
Appearance
Dimensions (W×D×H)	235 mm × 385 mm × 175 mm
Weight	5.6 kg / 5.7 kg / 5.8 kg
Electrical Parameters
Power	~220 V / 50 Hz, 255 W
Data Interface	USB 2.0 × 2 (two on the right side)
Environmental Parameters
Operating Conditions	Temperature: 10–30 °C, Humidity: 20%–80%
Transportation & Storage Conditions	Temperature: –20–55 °C, Humidity: 20%–80%
Altitude	< 2500 meters
Noise Level	A-weighted, < 60 dB
Sample Parameters
Sample Capacity	16 × 0.2 mL / 32 × 0.2 mL / 48 × 0.2 mL
Tube Type	Single tube, 8-strip tube
Sample Volume	15–100 μL
Temperature Characteristics
Heating/Cooling Method	Semiconductor heating/cooling
Temperature Range	4 °C–99 °C
Max. Heating Rate	≥ 3.5 °C/s
Avg. Heating Rate	≥ 2.5 °C/s
Max. Cooling Rate	≥ 3.5 °C/s
Avg. Cooling Rate	≥ 2.5 °C/s
Temperature Control Accuracy	≤ ±0.01 °C
Temperature Accuracy	≤ ±0.1 °C
Temperature Uniformity	≤ ±0.3 °C
Optical Characteristics
Number of Fluorescence Detection Channels	2 / 4 / 4
Light Emitting Device	High-brightness LED
Light Collection Device	High-sensitivity, high signal-to-noise ratio photodiode
Compatible Probes/Dyes
Channel 1	470/520: FAM, SYBR Green
Channel 2	530/570: HEX, JOE, VIC
Channel 3	580/610: ROX, Cy3.5, Texas Red
Channel 4	630/670: Cy5
Detection Sensitivity	1 copy
Linear Detection Range	10⁰–10¹⁰ copies
Linear Correlation Coefficient	≥ 0.999
Channel Crosstalk	No crosstalk
Detection Repeatability	≤ 1.0%