I. Product Overview

It performs rapid and accurate qualitative and quantitative analysis of target nucleic acids in biological samples taken from other animals (such as blood, body fluids, etc.), and can also analyze special samples for standard curves, melting curves, genotyping, etc.

II. Basic Principle

Real-time fluorescent quantitative PCR technology adds corresponding fluorescent dyes or fluorescent-labeled probes on the basis of conventional PCR. During the PCR reaction, the entire PCR process is detected in real time through changes in fluorescent signals. It is a method of monitoring the total product after each PCR cycle with fluorescent chemicals, and quantitatively analyzing specific DNA sequences in the test sample by internal or external reference methods.

III. Basic Instrument Structure

The Fluorescent Quantitative PCR Detector is a real-time reaction detection instrument, mainly composed of a gene amplification thermal cycling system, a real-time fluorescence detection system, a microcircuit control system, a computer and supporting application software. It has two core functional modules: the thermal cycling system and the real-time fluorescence detection system. The working principle of the gene amplification thermal cycling system is basically the same as that of traditional gene amplifiers, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly comprises fluorescence excitation components, optical signal transmission components, fluorescence detection components and a control system.

IV. Application Fields

• Basic scientific research

• Pathogen detection

• Animal epidemic detection

• Meat product adulteration

• GMO detection

• Food hygiene and quarantine

• Drug development and rational drug use

• Gene expression

• Water monitoring

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V. Equipment Parameters