PRODUCTS

ContentUS

Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

Current location：HOME>PRODUCT>Specialized Instruments>African Swine Fever (ASF) Detector

African Swine Fever (ASF) DetectorYP-P48

【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of African Swine Fever (ASF) Detector, with custom services. Complete African Swine Fever (ASF) Detector models, high detection accuracy, trusted by customers.

+86 19963093538

Product Details

详情新_01

详情新_02

详情新_03

详情新_04

详情新_05

详情新_06

详情新_07

详情新_08

详情新_09

详情新_10

详情新_11

详情新_12

详情新_13

详情新_14

I. Product Overview

The YP‑P48 PCR Amplifier is mainly designed for real‑time fluorescent quantitative PCR (qPCR) experiments. It monitors the reaction in real time through fluorescence excitation and signal acquisition, plots fluorescence curves displayed on the instrument interface, performs data fitting and analysis, and finally generates experimental reports in PDF format.

It provides rapid, accurate qualitative and quantitative analysis of target nucleic acids in biological samples taken from animals (e.g., blood, body fluids). It also supports advanced analyses including standard curves, melting curves, and genotyping for specialized samples.

II. Basic Principles

Working Principle of the YP‑P48 PCR Amplifier

Polymerase Chain Reaction (PCR) is a molecular biology technique that amplifies specific DNA fragments in vitro. It can be regarded as a specialized DNA amplification process outside a living organism, consisting of repeated thermal cycles:

High‑temperature denaturation
Low‑temperature annealing
Moderate‑temperature extension

This cycle enables rapid amplification of target genes with high specificity, high sensitivity, simplicity, and speed, representing a major innovation in gene amplification technology. PCR can amplify trace amounts of target DNA by millions of times, greatly enhancing the ability to analyze and detect DNA molecules.

Real‑time fluorescent quantitative PCR (qPCR) is developed based on conventional PCR by adding fluorescent dyes or fluorescently labeled probes. It monitors the entire PCR process in real time by measuring fluorescence signal intensity after each cycle. Using internal or external references, it enables quantitative analysis of specific DNA sequences in test samples.

III. Basic Instrument Structure

The real‑time PCR amplifier consists of four main components:

Gene Amplification Thermal Cycler System
Real‑time Fluorescence Detection System
Microcircuit Control System
Computer & Application Software

The two core functional modules are:

Thermal Cycler System: Uses semiconductor heating/cooling to perform thermal cycling, with a working principle similar to traditional PCR amplifiers.
Real‑time Fluorescence Detection System: Composed of a fluorescence excitation unit, optical signal transmission components, fluorescence detection unit, and control system.

IV. Device Specifications

Item	YP‑P16	YP‑P32	YP‑P48
Mechanical
Dimensions (W×D×H)	235 mm × 385 mm × 175 mm	235 mm × 385 mm × 175 mm	235 mm × 385 mm × 175 mm
Weight	5.6 kg	5.7 kg	5.8 kg
Electrical
Power	~220 V / 50 Hz, 255 W	~220 V / 50 Hz, 255 W	~220 V / 50 Hz, 255 W
Data Interface	2 × USB 2.0 (right side)	2 × USB 2.0 (right side)	2 × USB 2.0 (right side)
Environmental
Operating Conditions	10–30 ℃, 20%–80% RH	10–30 ℃, 20%–80% RH	10–30 ℃, 20%–80% RH
Storage/Transport	−20–55 ℃, 20%–80% RH	−20–55 ℃, 20%–80% RH	−20–55 ℃, 20%–80% RH
Altitude	< 2500 m	< 2500 m	< 2500 m
Noise	A‑weighted, < 60 dB	A‑weighted, < 60 dB	A‑weighted, < 60 dB
Sample
Sample Capacity	16 × 0.2 mL	32 × 0.2 mL	48 × 0.2 mL
Tube Type	Single tubes, 8‑tube strips	Single tubes, 8‑tube strips	Single tubes, 8‑tube strips
Reaction Volume	15–100 μL	15–100 μL	15–100 μL
Thermal Performance
Heating/Cooling	Semiconductor	Semiconductor	Semiconductor
Temperature Range	4 ℃ – 99 ℃	4 ℃ – 99 ℃	4 ℃ – 99 ℃
Max Heating Rate	≥ 3.5 ℃/s	≥ 3.5 ℃/s	≥ 3.5 ℃/s
Avg Heating Rate	≥ 2.5 ℃/s	≥ 2.5 ℃/s	≥ 2.5 ℃/s
Max Cooling Rate	≥ 3.5 ℃/s	≥ 3.5 ℃/s	≥ 3.5 ℃/s
Avg Cooling Rate	≥ 2.5 ℃/s	≥ 2.5 ℃/s	≥ 2.5 ℃/s
Temperature Precision	≤ ±0.01 ℃	≤ ±0.01 ℃	≤ ±0.01 ℃
Temperature Accuracy	≤ ±0.1 ℃	≤ ±0.1 ℃	≤ ±0.1 ℃
Temperature Uniformity	≤ ±0.3 ℃	≤ ±0.3 ℃	≤ ±0.3 ℃
Optical Performance
Fluorescence Channels	2	4	4
Light Source	High‑brightness LED	High‑brightness LED	High‑brightness LED
Detector	High‑sensitivity, high‑SNR photodiode	High‑sensitivity, high‑SNR photodiode	High‑sensitivity, high‑SNR photodiode
Supported Probes/Dyes	Channel 1: 470/520 FAM, SYBR Green Channel 2: 530/570 HEX, JOE, VIC	Channel 1: 470/520 FAM, SYBR Green Channel 2: 530/570 HEX, JOE, VIC Channel 3: 580/610 ROX, Cy3.5, Texas Red Channel 4: 630/670 Cy5	Channel 1: 470/520 FAM, SYBR Green Channel 2: 530/570 HEX, JOE, VIC Channel 3: 580/610 ROX, Cy3.5, Texas Red Channel 4: 630/670 Cy5
Detection Sensitivity	1 copy	1 copy	1 copy
Linear Range	10⁰ – 10¹⁰ copies	10⁰ – 10¹⁰ copies	10⁰ – 10¹⁰ copies
Correlation Coefficient	≥ 0.999	≥ 0.999	≥ 0.999
Channel Crosstalk	None	None	None
Repeatability	≤ 1.0%	≤ 1.0%	≤ 1.0%