PRODUCTS

ContentUS

Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

Current location：HOME>PRODUCT>Specialized Instruments>Pine Wood Nematode Detector

Pine Wood Nematode DetectorYP-P48

【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of Pine Wood Nematode Detector, with custom services. Complete Pine Wood Nematode Detector models, high detection accuracy, trusted by customers.

+86 19963093538

Product Details

Instrument Overview

The Pine Wood Nematode Detector is designed for the detection of Pine Wood Nematode (Bursaphelenchus xylophilus), a highly destructive plant‑parasitic nematode that causes severe wilt disease and mortality in pine trees (especially Pinaceae species). Early detection and monitoring are critical for implementing timely control measures and minimizing the impact of epidemics.

Detection Procedure

The Pine Wood Nematode Detector is typically based on molecular biological techniques, consisting of the following steps:

Sample Collection
Collect samples from suspected infected pine trees or wood materials, including trunks, bark, roots, sawdust, or other plant tissues where nematodes may be present.
DNA Extraction
Extract genomic DNA of the pine wood nematode from the samples using appropriate methods to obtain genetic material for subsequent molecular detection.
qPCR Amplification
Perform specific amplification of pine wood nematode DNA using real‑time quantitative polymerase chain reaction (qPCR) to determine the presence of the nematode and quantify the initial copy number of the target DNA sequence.

Instrument Structure

The Pine Wood Nematode Detector is a real‑time reaction monitoring instrument, mainly composed of:

Gene Amplification Thermal Cycler System
Real‑time Fluorescence Detection System
Microcircuit Control System
Computer and application software

The two core functional modules are the thermal cycler system and the real‑time fluorescence detection system.

The gene amplification thermal cycler uses semiconductor heating and cooling to perform thermal cycling, with a working principle similar to conventional PCR instruments.
The fluorescence detection system comprises a fluorescence excitation unit, optical signal transmission components, a fluorescence detection unit, and a control system.

Device Specifications

Item	Specification
Mechanical
Dimensions (W×D×H)	235 mm × 385 mm × 175 mm
Weight	5.8 kg
Electrical
Power	~220 V / 50 Hz, 255 W
Data Interface	2 × USB 2.0 (right side)
Environmental
Operating Conditions	10–30 ℃, 20%–80% RH
Storage & Transport	−20–55 ℃, 20%–80% RH
Altitude	< 2500 m
Noise	A‑weighted, < 60 dB
Sample
Sample Capacity	48 × 0.2 mL
Tube Type	Single tubes, 8‑tube strips, 16‑well blocks
Reaction Volume	15–100 μL
Thermal Performance
Heating/Cooling	Semiconductor heating / cooling
Temperature Range	4 ℃ – 99 ℃
Max Heating Rate	≥ 3.5 ℃/s
Avg Heating Rate	≥ 2.5 ℃/s
Max Cooling Rate	≥ 3.5 ℃/s
Avg Cooling Rate	≥ 2.5 ℃/s
Temperature Precision	≤ ±0.01 ℃
Temperature Accuracy	≤ ±0.1 ℃
Temperature Uniformity	≤ ±0.3 ℃
Optical Performance
Fluorescence Channels	4
Light Source	High‑brightness LED
Detector	High‑sensitivity, high‑SNR photodiode
Supported Probes/Dyes	Channel 1: 470/520 – FAM, SYBR Green Channel 2: 530/570 – HEX, JOE, VIC Channel 3: 580/610 – ROX, Cy3.5, Texas Red Channel 4: 630/670 – Cy5
Detection Sensitivity	1 copy
Linear Range	10⁰ – 10¹⁰ copies
Correlation Coefficient	≥ 0.999
Channel Crosstalk	None
Repeatability	≤ 1.0%